Coding

Part:BBa_K4242000:Design

Designed by: zhelin Jiang   Group: iGEM22_CUG-China   (2022-07-12)


FleQ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 868
    Illegal XhoI site found at 1321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 261
    Illegal NgoMIV site found at 1269
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1357
    Illegal NgoMIV site found at 1442
    Illegal AgeI site found at 670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 283
    Illegal SapI.rc site found at 1216
    Illegal SapI.rc site found at 1225


Design Notes

FleQ is is a protein that is a transcription factor.

Source

Pseudomonas aeruginosa, sequence found through Genbank https://www.ncbi.nlm.nih.gov/gene/881866

References

[1]Matsuyama BY, Krasteva PV, Baraquet C, Harwood CS, Sondermann H, Navarro MV. Mechanistic insights into c-di-GMP-dependent control of the biofilm regulator FleQ from Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):E209-18.

[2]Hickman JW, Harwood CS. Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor. Mol Microbiol. 2008 Jul;69(2):376-89.

[3]Baraquet C, Murakami K, Parsek MR, Harwood CS. The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP. Nucleic Acids Res. 2012 Aug;40(15):7207-18.